CERULOPLASMIN Test. lab information about test and diagnosis of diseases

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Ceruloplasmin Test. Lab information about test and diagnosis of diseases. 

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Ceruloplasmin Test – Manual of Medical Laboratory Techniques

Purpose:
To measure the amount of ceruloplasmin in human serum. Low levels are reported in Wilson’s disease and nephrotic syndrome, and it is valuable in differentiating chronic liver diseases from Wilson’s disease.

Principle:
Ceruloplasmin, a ferro-oxidase enzyme, catalyzes the oxidation of certain polyamines. Its activity on p-phenylenediamine is measured to determine the amount of ceruloplasmin in serum.

Performance Specifications:

• Linearity: up to 60 mg/dL
• Measurement range: 16–60 mg/dL
• Sensitivity: 16 mg/dL

Primary Sample:

• Use serum as the specimen.
• Collect 4 mL of venous blood in a plain red vacutainer.
• Allow to clot for 30 minutes, then centrifuge at 2500 rpm for 10 minutes.
• Avoid hemolyzed or turbid samples.
• Analyze within 3 hours of collection, or store serum at –20°C for up to 7 days.

Reagents and Consumables:

• p-Phenylenediamine hydrochloride: 5 g in 1 L distilled water (purified and crystallized).
• Acetic acid (1 M): 60 mL glacial acetic acid diluted to 1 L.
• Sodium acetate (1 M): 136 g per liter of water.
• Acetate buffer (400 mM, pH 5.5): Prepared by mixing acetic acid and sodium acetate.
• Sodium azide or sodium fluoride: Preservatives (5 g/L or 20 g/L).

Instrument:
Spectrophotometer (read at 530 nm).

Procedure:

Calculation:

Reference Range:

• Normal: 20 – 40 mg/dL

Critical/Alert Values:

• Not applicable.

Potential Sources of Error:

• Hemolyzed serum may give falsely elevated values.
• Samples not analyzed promptly must be stored at –20°C to maintain accuracy.

References:
Ramakrishnan, Manual of Medical Laboratory Techniques, pp. 25–27.
King, J. Practical Clinical Enzymology, 1965.

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Produce by : Dr. Yousef AL-Adbai.